| Autor: |
Jingxin Wang, Wenbo Pan, Alexiy Nikiforov, William King, Wanting Hong, Weiwei Li, Yang Han, Jana Patton-Vogt, Jianbo Shen, Lingyun Cheng |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Crop Journal, Vol 9, Iss 1, Pp 95-108 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2214-5141 |
| DOI: |
10.1016/j.cj.2020.05.004 |
| Popis: |
Phosphate deficiency is one of the leading causes of crop productivity loss. Phospholipid degradation liberates phosphate to cope with phosphate deficiency. Glycerophosphodiester phosphodiesterases (GPX-PDEs) hydrolyse the intermediate products of phospholipid catabolism glycerophosphodiesters into glycerol-3-phosphate, a precursor of phosphate. However, the function of GPX-PDEs in phosphate remobilization in maize remains unclear. In the present study, we characterized two phosphate deficiency-inducible GPX-PDE genes, ZmGPX-PDE1 and ZmGPX-PDE5, in maize leaves. ZmGPX-PDE1 and ZmGPX-PDE5 were transcriptionally regulated by ZmPHR1, a well-described phosphate starvation-responsive transcription factor of the MYB family. Complementation of the yeast GPX-PDE mutant gde1∆ indicated that ZmGPX-PDE1 and ZmGPX-PDE5 functioned as GPX-PDEs, suggesting their roles in phosphate recycling from glycerophosphodiesters. In vitro enzyme assays showed that ZmGPX-PDE1 and ZmGPX-PDE5 catalysed glycerophosphodiester degradation with different substrate preferences for glycerophosphoinositol and glycerophosphocholine, respectively. ZmGPX-PDE1 was upregulated during leaf senescence, and more remarkably, loss of ZmGPX-PDE1 in maize compromised the remobilization of phosphorus from senescing leaves to young leaves, resulting in a stay-green phenotype under phosphate starvation. These results suggest that ZmGPX-PDE1 catalyses the degradation of glycerophosphodiesters in maize, promoting phosphate recycling from senescing leaves to new leaves. This mechanism is crucial for improving phosphorus utilization efficiency in crops. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
