Relationships Between Disc Degeneration and Autophagy Expression in Human Nucleus Pulposus

Autor:	Meiling Quan, Myoung‐Wha Hong, Myung‐Sup Ko, Young‐Yul Kim
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Autophagy Degenerative grade Disc degeneration LAMP LC3 Orthopedic surgery RD701-811
Zdroj:	Orthopaedic Surgery, Vol 12, Iss 1, Pp 312-320 (2020)
Druh dokumentu:	article
ISSN:	1757-7861 1757-7853
DOI:	10.1111/os.12573
Popis:	Objectives To elaborate on the relationship between degeneration grade and autophagy expression in human nucleus pulposus obtained from surgical procedures. Methods For the 16 patients included in the present study, we determined the Pfirrmann classifications of degenerative lesions by MRI. Western blot analysis, LC3, LAMP2, and p62 protein expressions were quantified in different degeneration grades of disc nucleus pulposus. Double immunofluorescence staining was used to show co‐localization of LC3 and LAMP2, and immunohistochemistry to show LC3 and p62 in the nucleus pulposus. Results In the western blot analysis, LC3‐II was highly expressed in grade III and decreased progressively from grade IV to V. In addition, LC3‐II expression in grade III was significantly higher than in grade II, IV, and V (P
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/497e01a64bc54be0bf2ee9c19a546369 Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
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