A validated restriction enzyme ddPCR cg05575921 (AHRR) assay to accurately assess smoking exposure

Autor: Sandra Fitzgerald, Basharat Bhat, Cristin Print, Gregory T. Jones
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Clinical Epigenetics, Vol 16, Iss 1, Pp 1-7 (2024)
Druh dokumentu: article
ISSN: 1868-7083
DOI: 10.1186/s13148-024-01659-1
Popis: Abstract Background & Methods In this study, a novel restriction enzyme (RE) digestion-based droplet digital polymerase chain reaction (ddPCR) assay was designed for cg005575921 within the AHRR gene body and compared with matching results obtained by bisulfite conversion (BIS) ddPCR and Illumina DNA methylation array. Results The RE ddPCR cg05575921 assay appeared concordant with BIS ddPCR (r 2 = 0.94, P
Databáze: Directory of Open Access Journals
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