Autor: |
Rahman Mohammad Sabri Abdul, Khor Kuan Hua, Khairani-Bejo Siti, Lau Seng Fong, Mazlan Mazlina, Roslan Mohd Azri, Ajat Mohd Mokrish Md, Noor Mohd Akmal Mohd |
Jazyk: |
angličtina |
Rok vydání: |
2023 |
Předmět: |
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Zdroj: |
Journal of Veterinary Research, Vol 67, Iss 2, Pp 187-195 (2023) |
Druh dokumentu: |
article |
ISSN: |
2450-8608 |
DOI: |
10.2478/jvetres-2023-0024 |
Popis: |
Canine leptospirosis has always been a differential diagnosis in dogs presenting with clinical signs and blood profiles associated with kidney and/or liver disease. The conventional polymerase chain reaction (PCR) provides diagnoses, but real-time PCR-based tests provide earlier confirmation and determine the severity of infection, especially in the acute stage, allowing early detection for immediate treatment decisions. To our knowledge, real-time PCR has not been routinely adopted for clinical investigation in Malaysia. This study evaluated TaqMan real-time PCR (qPCR) assays diagnosing leptospirosis and compared their applicability to clinical samples from dogs with kidney and/or liver disease against a conventional PCR reference. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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