TaqMan real-time PCR for detection of pathogenic Leptospira spp. in canine clinical samples

Autor: Rahman Mohammad Sabri Abdul, Khor Kuan Hua, Khairani-Bejo Siti, Lau Seng Fong, Mazlan Mazlina, Roslan Mohd Azri, Ajat Mohd Mokrish Md, Noor Mohd Akmal Mohd
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Journal of Veterinary Research, Vol 67, Iss 2, Pp 187-195 (2023)
Druh dokumentu: article
ISSN: 2450-8608
DOI: 10.2478/jvetres-2023-0024
Popis: Canine leptospirosis has always been a differential diagnosis in dogs presenting with clinical signs and blood profiles associated with kidney and/or liver disease. The conventional polymerase chain reaction (PCR) provides diagnoses, but real-time PCR-based tests provide earlier confirmation and determine the severity of infection, especially in the acute stage, allowing early detection for immediate treatment decisions. To our knowledge, real-time PCR has not been routinely adopted for clinical investigation in Malaysia. This study evaluated TaqMan real-time PCR (qPCR) assays diagnosing leptospirosis and compared their applicability to clinical samples from dogs with kidney and/or liver disease against a conventional PCR reference.
Databáze: Directory of Open Access Journals