Validation of the XTT-test for assessing the antiproliferative activity of biologics on the basis of monoclonal antibodies

Autor:	I. V. Lyagoskin, M. A. Berestovoy, D. A. Poteryaev, E. S. Zeinalova, A. Yu. Vishnevskiy, A. A. Kazarov
Jazyk:	ruština
Rok vydání:	2018
Předmět:	хтт вт-474 трастузумаб моноклональные антитела рак биопрепараты валидация специфичность линейность правильность прецизионность xtt bt-474 trastuzumab monoclonal antibodies cancer biologics validation specificity calibration curve accuracy precision Biotechnology TP248.13-248.65 Medicine
Zdroj:	Биопрепараты: Профилактика, диагностика, лечение, Vol 0, Iss 1, Pp 45-50 (2018)
Druh dokumentu:	article
ISSN:	2221-996X 2619-1156
Popis:	Using the example of Herceptin (Roche) a method of assessing the antiproliferative activity of monoclonal antibodies using tetrazolium salt was developed. The method is based on spectrometric measurement of the level of formazan transformed by living cells from a water soluble salt XTT. The level of formazan is proportional to the number of viable cells. The technique was in terms of specificity, calibration curve, accuracy and precision.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/493a6da5efee433e83f775eacdd45ac0 Zobrazit plný text záznamu View record in DOAJ