| Autor: |
Lenka Fáber, Ivan Kováč, Petra Mitrengová, Martin Novotný, Lenka Varinská, Tomáš Vasilenko, Martin Kello, Matúš Čoma, Tomáš Kuruc, Klaudia Petrová, Ivana Miláčková, Anika Kuczmannová, Vlasta Peržeľová, Štefánia Mižáková, Erik Dosedla, František Sabol, Ján Luczy, Milan Nagy, Jaroslav Majerník, Martin Koščo, Pavel Mučaji, Peter Gál |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Molecules, Vol 23, Iss 7, p 1637 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
1420-3049 |
| DOI: |
10.3390/molecules23071637 |
| Popis: |
Selective estrogen receptor modulators (SERMs) have been developed to achieve beneficial effects of estrogens while minimizing their side effects. In this context, we decided to evaluate the protective effect of genistein, a natural SERM, on skin flap viability in rats and in a series of in vitro experiments on endothelial cells (migration, proliferation, antioxidant properties, and gene expression profiling following genistein treatment). Our results showed that administration of genistein increased skin flap viability, but importantly, the difference is only significant when treatment is started 3 days prior the flap surgery. Based on our in vitro experiments, it may be hypothesized that the underlying mechanism may rather by mediated by increasing SOD activity and Bcl-2 expression. The gene expression profiling further revealed 9 up-regulated genes (angiogenesis/inflammation promoting: CTGF, CXCL5, IL-6, ITGB3, MMP-14, and VEGF-A; angiogenesis inhibiting: COL18A1, TIMP-2, and TIMP-3). In conclusion, we observed a protective effect of genistein on skin flap viability which could be potentially applied in plastic surgery to women undergoing a reconstructive and/or plastic intervention. Nevertheless, further research is needed to explain the exact underlying mechanism and to find the optimal treatment protocol. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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