| Autor: |
Shiliang Li, Yan Zhou, KunSheng Li, Lu Liu, Ming Fang, Hongfeng Gao |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
ESC Heart Failure, Vol 11, Iss 5, Pp 2730-2741 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2055-5822 |
| DOI: |
10.1002/ehf2.14809 |
| Popis: |
Abstract Aims This study aims to explore the molecular mechanism of circular RNAs' (circRNAs) potential involvement in myocardial ischaemia–reperfusion injury (MIRI). Methods and results Differently expressed genes in myocardial infarction (MI) were identified by screening the GEO database. Serum was collected from MI patients and healthy volunteers (n = 5 for each group). AC16 cells were cultured and exposed to hypoxia/reperfusion (H/R) treatment for the cell experiments. Then candidate genes were validated in human serum and the H/R model. Quantitative real‐time PCR and western blot were used to detect expression of key molecules such as circDGKZ, miR‐345‐5p, and Toll‐like receptor 4 (TLR4), as well as pyroptosis markers such as NOD‐like receptor thermal protein domain‐associated protein 3 (NLRP3), ASC, C‐caspase1, interleukin (IL)‐1β, and IL‐18. CircDGKZ was positively correlated in human serum (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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