Improvement of digital Gabor holographic microscopy using a lens in plankton studies

Autor: Yaumel C. Arias-Sosa, Gelaysi Moreno-Vega, Rubens M. Lopes, José-Luis Valin-Rivera, Meylí Valin-Fernández, Edison Gonçalves, Jorge O. Ricardo-Pérez
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Heliyon, Vol 10, Iss 9, Pp e29441- (2024)
Druh dokumentu: article
ISSN: 2405-8440
DOI: 10.1016/j.heliyon.2024.e29441
Popis: This work utilizes a Gabor Holographic Optical Scheme integrated with a microscope objective and a thin convex plane lens. This bi-telecentric lens system corrects spherical aberration from the objective, maintains consistent magnification across various reconstruction distances, and ensures a plane incidence on CMOS. Depending on the focal lengths of the objective and lens, the final image can be enlarged or reduced compared to the classic Gabor system, resulting in high-quality reconstructed phase images without spherical aberration. This setup was employed to capture phase distribution and intensity images of planktonic objects, such as copepods, achieving superior image quality.
Databáze: Directory of Open Access Journals