A simple pressure-assisted method for MicroED specimen preparation

Autor: Jingjing Zhao, Hongyi Xu, Hugo Lebrette, Marta Carroni, Helena Taberman, Martin Högbom, Xiaodong Zou
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Nature Communications, Vol 12, Iss 1, Pp 1-7 (2021)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-021-25335-7
Popis: Micro-crystal electron diffraction (MicroED) has shown great potential for the structure determination of crystals that are too small for X-ray diffraction but MicroED sample preparation remains challenging. Here, the authors present Preassis, a pressure-assisted method for the preparation of MicroED specimens and demonstrate that Preassis can be applied to a wide range of protein crystal suspensions with low and high viscosities, as well as those with low crystal concentrations.
Databáze: Directory of Open Access Journals