Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields

Autor: Mina Aleksanyan, Hammad A. Faizi, Maria-Anna Kirmpaki, Petia M. Vlahovska, Karin A. Riske, Rumiana Dimova
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Advances in Physics: X, Vol 8, Iss 1 (2023)
Druh dokumentu: article
ISSN: 23746149
2374-6149
DOI: 10.1080/23746149.2022.2125342
Popis: ABSTRACTKnowledge of the material properties of membranes is crucial to understanding cell viability and physiology. A number of methods have been developed to probe membranes in vitro, utilizing the response of minimal biomimetic membrane models to an external perturbation. In this review, we focus on techniques employing giant unilamellar vesicles (GUVs), model membrane systems, often referred to as minimal artificial cells because of the potential they offer to mimick certain cellular features. When exposed to electric fields, GUV deformation, dynamic response and poration can be used to deduce properties such as bending rigidity, pore edge tension, membrane capacitance, surface shear viscosity, excess area and membrane stability. We present a succinct overview of these techniques, which require only simple instrumentation, available in many labs, as well as reasonably facile experimental implementation and analysis.
Databáze: Directory of Open Access Journals
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