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Mohammad Hasan Moshafi,1 Mehdi Ranjbar,1 Ghazaleh Ilbeigi21Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran; 2Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, IranBackground: Biotemplates are attractive templates for the synthesis of nanometals and inorganic compound nanostructures.Methods: In this work, for the first time, iron oxide quantum dot nanoparticles (QDNPs) were prepared using egg albumen as a biotemplate. Next, the prepared nanoparticles were characterized using dynamic light scattering for determination and evaluation of the hydrodynamic diameter and zeta potential of the particles. Moreover, optical and scanning electron microscopes were applied to evaluate morphology. Spherically shaped iron oxide QDNPs were obtained with appropriate particle size and distribution. Fe(NO3)3.9H2O and egg whites were used as the source of the Fe element and particle size control agent in the aqueous medium, respectively. Afterward, the effect of calcination temperature parameters on the crystallinity purity and size of Fe nanocrystals were investigated. Also, products were characterized by various detection analyses such as thermogravimetry analysis/DTA, XRD, UV-vis, Fourier transform infrared (FT-IR,) transmission electron microscopy, and SEM. In order to investigate the antibacterial effect of the synthesized Fe nanobiological samples against bacterial strains, they were dissolved in dimethyl sulfoxide and diluted using distilled water. Then, different serial dilutions of 64 μg/mL, 32 μg/mL, 16 μg/mL, 8 μg/mL, 4 3BCg/mL, 2 μg/mL, 1 μg/mL, and 0.5 μg/mL of nanobiological samples were prepared and added to the Mueller–Hinton agar medium.Results: The minimum inhibitory concentration of the synthesized iron oxide quantum dot nanobiological was determined against pathogenic microbial strains of bacteria including Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, Micrococcus luteus, Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, and Klebsiella pneumonia on the culture medium plate.Conclusion: The present nanobiological samples can be considered as a new material candidate for antibacterial drugs.Keywords: iron oxide quantum dots, egg albumen, bio-template, antibacterial effect |
