Variability of filtration and food assimilation rates, respiratory activity and multixenobiotic resistance (MXR) mechanism in the mussel Perna perna under lead influence

Autor: M. L. PESSATTI, C. RESGALLA JR., R. W. REIS Fo., J. KUEHN, L. C. SALOMÃO, J. D. FONTANA
Jazyk: angličtina
Předmět:
Zdroj: Brazilian Journal of Biology, Vol 62, Iss 4a, Pp 651-656
Druh dokumentu: article
ISSN: 1678-4375
1519-6984
DOI: 10.1590/S1519-69842002000400013
Popis: The economic importance that myticulture is conquering in Santa Catarina State (South of Brazil) explains the crescent search for new coastal sites for farming. Physiological and biochemical studies of the mussel Perna perna are important to the establishment of methodologies for program assessment and environmental monitoring, allowing to infer about site quality and possible influences of xenobiotic agents on coastal areas. In order to evaluate effects caused by lead poisoning (1.21 mumol.L-1), the mussels were maintained at constant temperature (25ºC) and fed with Chaetoceros gracilis for 15 days. The control group was acclimatized in sea water 30‰. At the end of this period time, physiological measurements were carried out along with statistic analysis for filtration rates, lead assimilation and overall respiratory activity. The mechanism of multixenobiotic resistance (MXR) was particularly evaluated in standardized gill fragments using rhodamine B accumulation and its quantification under fluorescence optical microscopy. Regarding the control group, results had shown that the mussels maintenance in a lead-poisoned environment caused higher filtration rates (1.04 and 2.3 and L.h-1.g-1; p < 0.05) and lower assimilation rates (71.96% and 54.1%, respectively). Also it was confirmed a lesser rhodamine B accumulation in the assays under influence of lead, suggesting that this metal induces the MXR mechanism expression in mussel P. perna. These results indicate that such physiological and biochemical alterations in the mussels can modify the energy fluxes of its metabolism, resulting in possible problems on the coastal systems used as cultivating sites.
Databáze: Directory of Open Access Journals
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