3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy

Autor: Luca Pesce, Marina Scardigli, Vladislav Gavryusev, Annunziatina Laurino, Giacomo Mazzamuto, Niamh Brady, Giuseppe Sancataldo, Ludovico Silvestri, Christophe Destrieux, Patrick R. Hof, Irene Costantini, Francesco S. Pavone
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Communications Biology, Vol 5, Iss 1, Pp 1-12 (2022)
Druh dokumentu: article
ISSN: 2399-3642
DOI: 10.1038/s42003-022-03390-0
Popis: An optimized clearing approach, named SHORT, applied to thick sections of human cortical tissue allows reconstruction of large portions of the tissues using light-sheet fluorescence microscopy, by performing multicolor immunostaining and multirounds.
Databáze: Directory of Open Access Journals
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