Uji Fungsional dan Karakteristik Sel Punca Hematopoetik Hasil Isolasi dari Darah Tali Pusat Manusia Menggunakan Metode Modifikasi Unpad- Aster

Autor: Tono Djuwantono, Firman F. Wirakusumah, Leri Septiani, Ike Kristina, Devi Natalia, Danny Halim, Ahmad Faried
Jazyk: English<br />Indonesian
Rok vydání: 2011
Předmět:
Zdroj: Majalah Kedokteran Bandung, Vol 43, Iss 4, Pp 171-177 (2011)
Druh dokumentu: article
ISSN: 0126-074X
2338-6223
DOI: 10.15395/mkb.v43n4.65
Popis: The conventional method of mononuclear cells (MNCs) isolation from human umbilical cord blood (UCB) yielded high erythrocyte contamination level. Therefore, the aim of this study was to assess the differences of cell viability and erythrocyte contamination on the population of UCB MNCs in our modified isolation method. This study was also aimed to test the function and characteristic of human MNCs derived from UCB as the basis for the development of UCB banking in Indonesia. The study was conducted in Department of Obstetry and Ginecology >RS Dr. Hasan Sadikin Bandung in period of January–October 2010. The modified isolation method (namely Unpad Aster’s modification) yielded 5.1x106 MNC cell/mL has lower erythrocyte contamination level than conventional method. The morphology of MNCs cultured in unrestricted somatic stem cells (USSCs) medium looked like adhered cells (attached at the surface of culture flask), spindle-shaped cells with positive luster of ifferentiation-90 (CD-90) (leukocyte antigen) and cluster of differentiation-105 (CD-105) and could differentiate into neuronal cells and adipocytes. While the morphology of cord blood-derived multipotent progenitor cells (CB-MPCs) looked like fibroblast cells with positive cluster of differentiation-45 (CD-45) (antigen hematopoietic) and could differentiate into neuronal cells. In conclusions, the Unpad-Aster’s modified isolation method gives lower level of erythrocyte contamination compared with conventional method. Mononuclear cells derived from UCB could differentiate into neuronal cells and adipocytes.
Databáze: Directory of Open Access Journals
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