Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva
| Autor: | Patrícia Cuervo, Camila Mesquita-Rodrigues, Claudia Masini d'Avila Levy, Constança Britto, Fabiano Araújo Pires, Rodrigo Gredilha, Carlos Roberto Alves, Jose Batista de Jesus |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Memorias do Instituto Oswaldo Cruz, Vol 103, Iss 5, Pp 504-506 (2008) |
| Druh dokumentu: | article |
| ISSN: | 0074-0276 1678-8060 |
| DOI: | 10.1590/S0074-02762008000500018 |
| Popis: | We report for the first time the expression of multiple protease activities in the first instar larva (L1) of the flesh fly Oxysarcodexia thornax (Walker). Zymographic analysis of homogenates from freshly obtained L1 revealed a complex proteolytic profile ranging from 21.5 to 136 kDa. Although some activities were detected at pH 3.5 and 5.5, the optimum pH for most of the proteolytic activities was between pH 7.5 and 9.5. Seven of 10 proteases were completely inactivated by phenyl-methyl sulfonyl-fluoride, suggesting that main proteases expressed by L1 belong to serine proteases class. Complete inactivation of all enzymatic activities was obtained using N-p-Tosyl-L-phenylalanine chloromethyl ketone (100 µM), a specific inhibitor of chymotrypsin-like serine proteases. |
| Databáze: | Directory of Open Access Journals |
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