Single-cell quantification and dose-response of cytosolic siRNA delivery

Autor: Hampus Hedlund, Hampus Du Rietz, Johanna M. Johansson, Hanna C. Eriksson, Wahed Zedan, Linfeng Huang, Jonas Wallin, Anders Wittrup
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-023-36752-1
Popis: Endosomal escape and subsequent cytosolic delivery of siRNA therapeutics is inefficient, and quantification is difficult. Here the authors report a confocal microscopy-based method to quantify cytosolic delivery of fluorescently labelled siRNA during lipid-mediated delivery.
Databáze: Directory of Open Access Journals