Calorimetric Study of Helix aspersa Maxima Hemocyanin Isoforms

Autor: Svetla Todinova, Yuliana Raynova, Krassimira Idakieva
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Analytical Methods in Chemistry, Vol 2018 (2018)
Druh dokumentu: article
ISSN: 2090-8865
2090-8873
DOI: 10.1155/2018/8450792
Popis: The thermal unfolding of hemocyanin isoforms, β-HaH and αD+N-HaH, isolated from the hemolymph of garden snails Helix aspersa maxima, was studied by means of differential scanning calorimetry (DSC). One transition, with an apparent transition temperature (Tm) at 79.88°C, was detected in the thermogram of β-HaH in 20 mM HEPES buffer, containing 0.1 M NaCl, 5 mM CaCl2, and 5 mM MgCl2, pH 7.0, at scan rate of 1.0°C min−1. By means of successive annealing procedure, two individual transitions were identified in the thermogram of αD+N-HaH. Denaturation of both hemocyanins was found to be an irreversible process. The scan-rate dependence of the calorimetric profiles indicated that the thermal unfolding of investigated hemocyanins was kinetically controlled. The thermal denaturation of the isoforms β-HaH and αD+N-HaH was described by the two-state irreversible model, and parameters of the Arrhenius equation were calculated.
Databáze: Directory of Open Access Journals
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