iTRAQ-based comparative proteomic analysis of cells infected with Eimeria tenella sporozoites

Autor: Zhao Zongping, Zhao Qiping, Zhu Shunhai, Huang Bing, Lv Ling, Chen Ting, Yan Ming, Han Hongyu, Dong Hui
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Parasite, Vol 26, p 7 (2019)
Druh dokumentu: article
ISSN: 1776-1042
DOI: 10.1051/parasite/2019009
Popis: Eimeria tenella is an obligate intracellular parasite that actively invades cecal epithelial cells of chickens. When E. tenella infects a host cell, the host produces a corresponding change to deal with damage caused by this infection. To date, our knowledge on the mechanism of how the host cell responds to E. tenella infection is highly limited at both the molecular and cellular levels. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS was used to screen the differentially expressed proteins (DEPs) in BHK-21 cells infected with E. tenella sporozoites for 24 h post infection. In total, 6139 non-redundant distinct proteins were identified and 195 of these were found to have a fold change ratio ≥1.3 or ≤0.7 and p
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