| Autor: |
Yeounsun Oh, Wi-jae Lee, Junho K. Hur, Woo Jeung Song, Youngjeon Lee, Hanseop Kim, Lee Wha Gwon, Young-Hyun Kim, Young-Ho Park, Chan Hyoung Kim, Kyung-Seob Lim, Bong-Seok Song, Jae-Won Huh, Sun-Uk Kim, Bong-Hyun Jun, Cheulhee Jung, Seung Hwan Lee |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Genome Biology, Vol 23, Iss 1, Pp 1-12 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1474-760X |
| DOI: |
10.1186/s13059-022-02644-8 |
| Popis: |
Abstract Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas9, which is a CRISPR-Cas9 ortholog. Using FnCas9(H969A) nickase, the targeting limitation of existing Streptococcus pyogenes Cas9 nickase [SpCas9(H840A)]-based prime editing is dramatically extended, and accurate prime editing is induced specifically for the target genes in human cell lines. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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