Development of a Rat Model for Glioma-Related Epilepsy

Autor: Charlotte Bouckaert, Charlotte Germonpré, Jeroen Verhoeven, Seon-Ah Chong, Lucas Jacquin, Georges Mairet-Coello, Véronique Marie André, Karine Leclercq, Christian Vanhove, Filip De Vos, Caroline Van den Broecke, Ingeborg Goethals, Benedicte Descamps, Sam Donche, Evelien Carrette, Wytse Wadman, Paul Boon, Kristl Vonck, Robrecht Raedt
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: International Journal of Molecular Sciences, Vol 21, Iss 19, p 6999 (2020)
Druh dokumentu: article
ISSN: 1422-0067
1661-6596
DOI: 10.3390/ijms21196999
Popis: Seizures are common in patients with high-grade gliomas (30–60%) and approximately 15–30% of glioblastoma (GB) patients develop drug-resistant epilepsy. Reliable animal models are needed to develop adequate treatments for glioma-related epilepsy. Therefore, fifteen rats were inoculated with F98 GB cells (GB group) and four rats with vehicle only (control group) in the right entorhinal cortex. MRI was performed to visualize tumor presence. A subset of seven GB and two control rats were implanted with recording electrodes to determine the occurrence of epileptic seizures with video-EEG recording over multiple days. In a subset of rats, tumor size and expression of tumor markers were investigated with histology or mRNA in situ hybridization. Tumors were visible on MRI six days post-inoculation. Time-dependent changes in tumor morphology and size were visible on MRI. Epileptic seizures were detected in all GB rats monitored with video-EEG. Twenty-one days after inoculation, rats were euthanized based on signs of discomfort and pain. This study describes, for the first time, reproducible tumor growth and spontaneous seizures upon inoculation of F98 cells in the rat entorhinal cortex. The development of this new model of GB-related epilepsy may be valuable to design new therapies against tumor growth and associated epileptic seizures.
Databáze: Directory of Open Access Journals
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