| Popis: |
Objective To explore the curative and protective effects of exosomes derived from human umbilical cord mesenchymal stem cells(hUCMSC-Ex)on traumatic pancreatitis in rats. Methods Sixty grown male SD rats (200~250 g) were selected and randomly divided into four groups (n=15 of each group): control, traumatic pancreatitis (TP), TP+hUC-MSCs, and TP+hUCMSC-Ex groups. In the control group, the pancreas was gently turned over after laparotomy. Subsequently, the abdomen was closed and 1 mL normal saline was injected via the tail vein. In the TP group, a rat model of TP was established using a model impactor and 1 mL of normal saline was injected via the tail vein. hUC-MSCs or hUCMSC-Ex were injected via tail vein after modeling in TP+hUC-MSCs and TP+hUCMSC-Ex groups. The ultracentrifugation was used to obtain exosomes, and transmission electron microscopy was used to identify exosomes; HE staining was used for detecting the severity of damage of pancreatic tissue; The expression levels of amylase, lipase, pro-inflammatory factors and anti-inflammatory factors were detected by ELISA; Cell apoptosis was detected by TUNEL; The expression levels of apoptosis proteins Bax and Caspase-3 were detected by Western blotting. Results Compared with TP group, TP+hUC-MSCs group and TP+hUCMSC-Ex group had obvious lower score on pancreas histopathological scoring(P < 0.05); The concentrations of serum amylase and lipase were significantly decreased (P < 0.05); The expression levels of serum pro-inflammatory factors IL-6 and TNF-α were significantly decreased, while the expression levels of anti-inflammatory factors IL-10 and TGF-β were significantly increased (P < 0.05); The apoptotic index in the TP group was significantly higher than that of in the control group (P < 0.05), and the apoptotic index in the TP+hUC-MSCs group and TP+hUCMSC-Ex group was significantly lower than that in the TP group (P < 0.05). Compared with TP group, the expression levels of pancreatic apoptotic proteins Bax and Caspase-3 were significantly decreased in the TP+hUC-MSCs group and TP+hUCMSC-Ex group (P < 0.05). Conclusion The hUCMSC-Ex can inhibit the apoptosis of rat acinar cells and participate in the regeneration and repair of pancreatic tissue by controlling the systemic inflammatory reaction. |
