Extending the Collection Duration of Breath Samples for Enteric Methane Emission Estimation Using the SF6 Tracer Technique

Autor: John Koolaard, Grant Taylor, German Molano, Sarah MacLean, Edgar Sandoval, Roberto Gratton, Paula Juliarena, César Pinares-Patiño, José Gere, Karen Williams
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Zdroj: Animals, Vol 2, Iss 2, Pp 275-287 (2012)
Druh dokumentu: article
ISSN: 2076-2615
DOI: 10.3390/ani2020275
Popis: The daily sample collection protocol of the sulphur hexafluoride (SF6) tracer technique for the estimation of methane (CH4) emissions from ruminants may not be practical under extensive grazing systems. Here, under controlled conditions, we evaluated extended periods of sampling as an alternative to daily sample collections. Eight rumen-fistulated cows were housed and fed lucerne silage to achieve common daily feed intakes of 6.4 kg dry matter per cow. Following SF6 permeation tube dosing, eight sampling lines were fitted to the breath collection harness, so that a common gas mix was available to each line. Half of the lines collected samples into PVC yokes using a modified capillary system as commonly used in New Zealand (NZL), and half collected samples into stainless steel cylinders using a ball-bearing flow restrictor as used in Argentina (ARG), all within a 10-day time frame, either daily, across two consecutive 5-day periods or across one 10-day period (in duplicate). The NZL system had greater sampling success (97.3 vs. 79.5%) and yielded more consistent CH4 emission estimates than the ARG system. Emission estimates from NZL daily, NZL 5-day and NZL 10-day samplings were 114, 110 and 111 g d−1, respectively. Extended sample collection protocol may be feasible, but definitive evaluation of this alternative as well as sample collection systems is required under grazing situations before a decision on recommendation can be made.
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