Modelling the time evolution of active caspase-3 protein in the rat lens after in vivo exposure to ultraviolet radiation-B.
| Autor: | Nooshin Talebizadeh, Zhaohua Yu, Martin Kronschläger, Per Söderberg |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | PLoS ONE, Vol 9, Iss 9, p e106926 (2014) |
| Druh dokumentu: | article |
| ISSN: | 1932-6203 17985153 |
| DOI: | 10.1371/journal.pone.0106926 |
| Popis: | PURPOSE: To introduce a model for the time evolution of active caspase-3 protein expression in albino rat lens up to 24 hours after in vivo exposure to low dose UVR in the 300 nm wavelength region (UVR-300 nm). METHODS: Forty Sprague-Dawley rats were unilaterally exposed in vivo to 1 kJ/m2 UVR-300 nm for 15 minutes. At 0.5, 8, 16, and 24 hours after the UVR exposure, the exposed and contralateral not-exposed lenses were removed and processed for immunohistochemistry. The differences in the probability of active caspase-3 expression at four different time points after exposure were used to determine the time evolution of active caspase-3 expression. A logistic model was introduced for the expression of active caspase-3. The parameters for the exposed and the not exposed lenses were estimated for the observation time points. RESULTS: The exposure to UVR-300 nm impacted on the parameters of the logistic model. Further, the parameters of the model varied with time after exposure to UVR-300 nm. CONCLUSION: The logistic model predicts the impact of exposure to UVR-300 nm on the spatial distribution of probability of active caspase-3 protein expression, depending on time. |
| Databáze: | Directory of Open Access Journals |
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