| Autor: |
Taghrid M. Gaafar, Rabab El Hawary, Amira Osman, Wael Attia, Hala Hamza, Konrad Brockmeier, Omneya M. Osman |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Middle East Fertility Society Journal, Vol 19, Iss 3, Pp 156-170 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
1110-5690 |
| DOI: |
10.1016/j.mefs.2014.01.002 |
| Popis: |
Objectives: Isolation and comparison of cellular and molecular characteristics of MSCs derived from full term amniotic membrane (AM), endometrium and ovarian tissues. Investigation of a possible therapeutic potential for AM MSCs in the female reproductive tract. Study design & methods: Human amniotic membranes (AMs) from term placentas (N = 8), endometrial/stromal (by scrapping endometrium) and ovarian tissues from premenopausal hysterectomies (N = 6) after mincing and enzymatic digestion were included in the study. Immunophenotyping by flow cytometry, Karyotyping, differentiation to mesoderm, RT-PCR analysis for transcription factors, WNT signaling pathways, pluripotency markers and HOXA genes were performed. Results: Generated cells showed morphologically and phenotypically the characteristics of MSCs from other sources with fairly comparable results. The cumulative population doubling was significantly higher in endometrium MSCs 25 ± 5.2 compared to 17.2 ± 4.5 for AM MSCs and 10.7 ± 2.4 for ovarian MSCs (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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