Morphological characteristics and cell viability of coffee plants calli
| Autor: | Marlúcia Souza Pádua, Luciano Vilela Paiva, Luciano Coutinho da Silva, Kalynka Gabriella do Livramento, Eduardo Alves, Ana Hortência Fonseca Castro |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Ciência Rural, Vol 44, Iss 4, Pp 660-665 (2014) |
| Druh dokumentu: | article |
| ISSN: | 1678-4596 0103-8478 |
| DOI: | 10.1590/S0103-84782014000400014 |
| Popis: | The aim of this research was to characterize and compare two types of calli from leaf explants of Coffea arabica (cultivar Catiguá). Cells of different types of callus were successfully characterized regarding viability and internal and external morphological characteristics. It was obtained two morphologically distinct types of callus: (i) yellow friable and (ii) transparent watery. The yellow friable calli showed higher cell viability and embryogenic characteristics. Scanning and transmission electron microscopy showed embryogenic characteristics in cells of the yellow friable calli evidenced by the presence of small and isodiametric cells, while transparent watery calli showed elongated cells and large cytoplasm vacuolization. |
| Databáze: | Directory of Open Access Journals |
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