Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Autor: Michail Michailidis, Evangelos Karagiannis, Georgia Tanou, Eirini Sarrou, Katerina Karamanoli, Athina Lazaridou, Stefan Martens, Athanassios Molassiotis
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Plant Methods, Vol 16, Iss 1, Pp 1-14 (2020)
Druh dokumentu: article
ISSN: 1746-4811
DOI: 10.1186/s13007-020-00593-6
Popis: Abstract Background Rain-induced fruit cracking is a major physiological problem in most sweet cherry cultivars. For an in vivo cracking assay, the ‘Christensen method’ (cracking evaluation following fruit immersion in water) is commonly used; however, this test does not adequately simulate environmental conditions. Herein, we have designed and evaluated a cracking protocol, named ‘Waterfall method’, in which fruits are continuously wetted under controlled conditions. Results The application of this method alone, or in combination with ‘Christensen method, was shown to be a reliable approach to characterize sweet cherry cracking behavior. Seventeen cherry cultivars were tested for their cracking behavior using both protocols, and primary as well as secondary metabolites identification was performed in skin tissue using a combined GC–MS and UPLC-MS/MS platform. Significant variations of some of the detected metabolites were discovered and important cracking index–metabolite correlations were identified. Conclusions We have established an alternative/complementary method of cherry cracking characterization alongside to Christiansen assay.
Databáze: Directory of Open Access Journals
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