| Autor: |
T. T. Nguyen, P. J. Dix, G. D. Nugent |
| Jazyk: |
angličtina |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Biologia Plantarum, Vol 54, Iss 1, Pp 141-144 (2010) |
| Druh dokumentu: |
article |
| ISSN: |
1573-8264 |
| DOI: |
10.1007/s10535-010-0022-2 |
| Popis: |
The transformation of potato (Solanum tuberosum L. cv. Désirée) was extended by the Agrobacterium-mediated biolistic method. Using this approach transgenic shoots could be obtained at a similar frequency to that achieved through conventional biolistics. Leaves from shoot cultures were bombarded with gold particles coated in Agrobacterium tumefaciens cells harboring a binary plasmid encoding three genes of interest in the T-DNA. Nine shoots were obtained from 20 shots, with selection of transgenic shoots on a series of media containing progressively increasing concentrations of hygromycin from 5 to 20 mg dm-3. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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