Allelic Variants of NRF2 and TLR9 Genes in Critical Illness
| Autor: | A. G. Chumachenko, A. E. Myazin, A. N. Kuzovlev, A. M. Gaponov, A. V. Tutelyan, L. N. Porokhovnik, A. M. Golubev, V. M. Pisarev |
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| Jazyk: | English<br />Russian |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Общая реаниматология, Vol 12, Iss 4, Pp 8-23 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1813-9779 2411-7110 |
| DOI: | 10.15360/1813-9779-2016-4-8-23 |
| Popis: | Aim of the study. To elucidate the association of allelic variants of single nucleotide polymorphism in NRF2 (rs6726395, 177238501A>G) and TLR9 (rs352162, 52218953T>C) genes, each gene separately and in their combination, with peculiarities of the course of critical conditions during lung infection. Materials and methods. DNA from 86 post#operative patients and oncologic patients was genotyped in an allelespecific fashion using tetra#primer polymerase chain reaction followed by gel electrophoresis analysis of products.Results. It has been found that septic shock patients with NRF2 177238501A>G GG genotype had increased mortality and higher APACHE II score and developed non#responsive edema more frequently. Patients with NRF2 177238501A>G GG/TLR9 52218953T>C CC genotype combination developed septic shock and nosocomialpneumonia more rarely.Conclusion. The homozygous NRF2 177238501A>G (GG) allele combination is unfavorable for the course and outcome of critical conditions only in combination with TLR9 52218953T>C СT or TLR9 52218953T>C TT alleles in septic shock patients. At the same time, the combination of TLR9 52218953T>C СС alleles in the same patients with 'unfavorable' NRF2 177238501A>G GG protects against development of septic shock and nosocomial pneumonia. |
| Databáze: | Directory of Open Access Journals |
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