The Expression of Matrix Metalloproteinases Activated Differently on In-Vitro Maturation of oocytes Cytoplasm and Cumulus Cells in Bovine
| Autor: | Sang-Hwan Ki, Jong-Taek Yoon |
|---|---|
| Jazyk: | English<br />Korean |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Journal of Animal Reproduction and Biotechnology, Vol 33, Iss 3, Pp 99-105 (2018) |
| Druh dokumentu: | article |
| ISSN: | 2671-4639 2671-4663 |
| DOI: | 10.12750/JET.2018.33.3.99 |
| Popis: | To determine the differences in the in-vitro ovum maturation process of bovine, we compared the expression of MMPs in these oocytes and cumulus cell throughout oocytes maturated. In an attempt to investigate the effect of MMP activation and inhibitors in total protein of cumulus cell and, oocytes during oocytes maturation, we examined and monitored the localization and expression of MMPs (MMP-2 and MMP-9), TIMPs (TIMP-2 and TIMP-3), as well as their expression profiles (Real-time PCR, Gelatin Zymography and ELISA). Our results that the bovine oocytes MMP-2 and MMP-9 level was significantly associated with the rate of maturity of oocytes (P |
| Databáze: | Directory of Open Access Journals |
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