| Autor: |
A. M. Grigoriev, Yu. B. Basok, A. D. Kirillova, L. A. Kirsanova, N. P. Shmerko, A. M. Subbot, E. A. Nemets, I. A. Miloserdov, M. Yu. Shagidulin, V. I. Sevastyanov |
| Jazyk: |
ruština |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Vestnik Transplantologii i Iskusstvennyh Organov, Vol 22, Iss 3, Pp 123-133 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1995-1191 |
| DOI: |
10.15825/1995-1191-2020-3-123-133 |
| Popis: |
Shortage of donor organs for liver transplantation in the treatment of end-stage liver disease dictates the need to develop alternative methods that include technologies on tissue engineering and regenerative medicine. Objective: to study the ability of a tissue-specific matrix from decellularized human liver fragments (DHLF) to maintain adhesion and proliferation of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and HepG2 under static conditions and in a flow-through bioreactor. Materials and methods. Treatment with surfactants (SAS) – sodium dodecyl sulfate, Triton X-100 – followed by exposure to DNase was used for decellularization of human liver fragments (no more than 8 mm3). Biochemical screening included the determination of DNA quantity in the test samples. Efficiency of surfactant washing was assessed by the cytotoxicity of the matrix in the NIH 3T3 fibroblast culture. Viability and metabolic activity of cells were assessed via vital staining with a complex of fluorescent dyes LIVE/DEAD ® and PrestoBlue™ (Invitrogen, USA). Morphological examination of the liver cell-engineered constructs was carried out through histological staining and scanning electron microscopy with lanthanide contrast. Results. It was shown that the liver decellularization method used allows to obtain a biocompatible matrix with a residual DNA quantity |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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