Minimally invasive method for murine brain fixation
| Autor: | Kenneth D. Eichenbaum, Joseph W. Eichenbaum, Ahmed Fadiel, Douglas C. Miller, Necdet Demir, Frederick Naftolin, Arnold Stern, Paul H. Pevsner |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 39, Iss 4, Pp 487-490 (2005) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/000112003 |
| Popis: | Complete brain fixation can be achieved with transthoracic cardiac infusion without thoracotomy. Light and electron microscopy tissue sections reveal preservation of cytoplasmic and nuclear structure at all magnification levels. Punched samples were obtained from the fixed tissue specimens in precisely localized areas for study using electron microscopy. This perfusion fixation technique provides both faster tissue harvesting capability and higher quality tissue preservation, without the artifacts of brain swelling and ventricular dilation observed in direct cardiac perfusion. Acute, discrete change in brain tissue can be studied. |
| Databáze: | Directory of Open Access Journals |
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