| Autor: |
Stephanie B. Hatch, Remko Prevo, Tiffany Chan, Val Millar, Bart Cornelissen, Geoff Higgins, Daniel Ebner |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 3, Iss 2, Pp 101355- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2022.101355 |
| Popis: |
Summary: The colony formation assay is the gold-standard technique to assess cell viability after treatment with cytotoxic reagents, ionizing radiation, and cytotoxic combinatorial treatments. This protocol describes a high-throughput automated and high-content imaging approach to screen siRNA molecular libraries in HeLa cervical cancer cells in 96-well format. We detail reverse transfection of cells with siRNAs, followed by ionizing radiation, fixing, and staining of the plates for automated colony counting. This protocol can be used across a broad range of cell types.For complete details on the use and execution of this protocol, please refer to Tiwana et al. (2015). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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