Therapeutic Effects of 17β-Estradiol on Pelvic Organ Prolapse by Inhibiting Mfn2 Expression: An In Vitro Study
| Autor: | Xiao-Qing Wang, Rui-Ju He, Bing-Bing Xiao, Ye Lu |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Frontiers in Endocrinology, Vol 11 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1664-2392 23785918 |
| DOI: | 10.3389/fendo.2020.586242 |
| Popis: | ObjectiveTo assess the effects of 17β-estradiol (E2) on proliferation, apoptosis, and protein expressions of fibroblasts at different concentrations and time intervals to reveal the mechanism of E2 in the treatment of pelvic organ prolapse (POP).Study DesignThe uterosacral ligament fibroblasts were collected from seven POP patients for primary culture of fibroblasts. The culture media containing 0, 10-6, 10-7, 10-8, and 10-9 mol/L E2 were used for 24, 48, 72, and 96 h.Main Outcome MeasuresThe cells were collected for cell counting kit-8 (CCK-8), apoptosis, quantitative reverse transcription polymerase chain reaction (qRT-PCR), and Western blotting assays.ResultsCompared with the control group, in the values of fibroblasts cultured in 10-8 mol/L E2 for 72 h, the proliferation, mRNA and protein expression of Mitofusin-2 (Mfn2) separately increased (P < 0.05), decreased (P |
| Databáze: | Directory of Open Access Journals |
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