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IntroductionThis randomized controlled trial aimed to address a knowledge gap concerning the mechanisms responsible for the efficacy of gratitude interventions. Specifically, we investigated how various response expectancies (positive, ambiguous + negative, and no expectancy) impact the efficacy of the “counting blessings” intervention in influencing positive and negative emotions. Additionally, the study explores how optimism levels (high, medium, low) interact with these expectancies to influence intervention efficacy.MethodA total of 529 adult volunteers were recruited through social media and randomly assigned to one of three experimental conditions, Positive Condition (PC), Ambiguous + Negative Condition (ANC), No Expectancy Condition (NEC), using a Random Sequence Generator. Of these, 142 participants completed the seven-day counting blessings intervention, and 111 participated in a follow-up assessment 1 month later. Missing data were addressed using multiple imputation. The main outcomes were changes in positive and negative emotions, with moderation analysis assessing the interaction between optimism levels and response expectancies. The study adhered to the CONSORT guidelines.ResultsWhile no significant interaction was found between experimental conditions and time regarding emotional outcomes (p ˃ 0.05), moderation analysis revealed differential interactions between optimism levels and expectancies, particularly influencing positive emotions (p |