Effect of citrus waste on blood parameters of broiler birds with and without cocktail of enzymes
Autor: | Devi Prasad Behera, Amrit Pal Singh Sethi, Chanchal Singh, Udeybir Singh, Manju Wadhwa |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: | |
Zdroj: | Veterinary World, Vol 12, Iss 4, Pp 483-488 (2019) |
Druh dokumentu: | article |
ISSN: | 0972-8988 2231-0916 |
DOI: | 10.14202/vetworld.2019.483-488 |
Popis: | Aim: This study aimed to assess the effect of different levels of citrus waste (CW) with or without enzyme cocktail on blood profile of broilers. Materials and Methods: CW was sun-dried and grounded to powder CW. 256-day-old birds were distributed into eight groups; control (C), CW-supplemented diets (2.5% CW, 5.0% CW, and 7.5% CW), enzyme (E) cocktail supplemented diets (CE, 2.5% CWE, 5.0% CWE, and 7.5% CWE). The diets were fed during starter (0-14 days), grower (15-21 days), and finisher (22-42 days) phases. Blood was collected from the wing vein from four birds per treatment. Serum was separated out after centrifugation and stored at −20°C until further analysis. The samples were analyzed for liver function test (glucose, total protein [TP], albumin [ALB], and globulin), lipid profile (cholesterol and triglyceride), kidney function test (alanine aminotransferase, aspartate aminotransferase [AST], blood urea nitrogen [BUN], and creatinine), and antioxidant levels (catalase, superoxide dismutase [SOD], lipid peroxidation [LPx], glutathione peroxidase [GPx], glutathione [GSH], and Vitamins E and C). Results: Blood profile data revealed that supplementation of CW showed no effect on TP, ALB, globulin, and BUN levels. Plasma cholesterol, triglyceride, and AST levels decreased linearly with an increase in the levels of CW in the diet. Catalase and SOD activity increased non-significantly with an increase in inclusion level of CW in the diets. LPx, GPx, and GSH activities decreased (p≤0.05) up to 5% CW-fed groups. Vitamin E and C activity were found to be highest (p≤0.05) in birds fed with diet supplemented with 5% CW. GPx and GSH activities and serum Vitamin C levels were observed to be highest (p |
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