Sulforaphane controls TPA-induced MMP-9 expression through the NF-κB signaling pathway, but not AP-1, in MCF-7 breast cancer cells
| Autor: | Young-Rae Lee, Eun-Mi Noh, Ji-Hey Han, Jeong-Mi Kim, Bo-Mi Hwang, Byeong-Soo Kim, Sung-Ho Lee, Sung Hoo Jung, Hyun Jo Youn, Eun Yong Chung, Jong-Suk Kim |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | BMB Reports, Vol 46, Iss 4, Pp 201-206 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1976-6696 1976-670X |
| DOI: | 10.5483/BMBRep.2013.46.4.160 |
| Popis: | Sulforaphane [1-isothiocyanato-4-(methylsulfinyl)-butane] is anisothiocyanate found in some cruciferous vegetables, especiallybroccoli. Sulforaphane has been shown to displayanti-cancer properties against various cancer cell lines. Matrixmetalloproteinase-9 (MMP-9), which degrades the extracellularmatrix (ECM), plays an important role in cancer cell invasion.In this study, we investigated the effect of sulforaphane on12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced MMP-9expression and cell invasion in MCF-7 cells. TPA-inducedMMP-9 expression and cell invasion were decreased bysulforaphane treatment. TPA substantially increased NF-κB andAP-1 DNA binding activity. Pre-treatment with sulforaphaneinhibited TPA-stimulated NF-κB binding activity, but not AP-1binding activity. In addition, we found that sulforaphanesuppressed NF-κB activation, by inhibiting phosphorylation ofIκB in TPA-treated MCF-7 cells. In this study, we demonstratedthat the inhibition of TPA-induced MMP-9 expression and cellinvasion by sulforaphane was mediated by the suppression ofthe NF-κB pathway in MCF-7 cells. [BMB Reports 2013; 46(4):201-206] |
| Databáze: | Directory of Open Access Journals |
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