| Autor: |
Diana Indrasanti, Aris Haryanto, Wayan T. Artama |
| Jazyk: |
English<br />Indonesian |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Molekul, Vol 6, Iss 1, Pp 10-18 (2011) |
| Druh dokumentu: |
article |
| ISSN: |
1907-9761 |
| Popis: |
Microneme protein (MIC) is one of proteins that belongs to excretory-secretory antigens (ESAs) of Toxoplasma gondii. Microneme 3 protein (MIC-3) is the protein that plays an important role in the invasion proccess during cell infection as a mediator attachment parasite to the host cell. The aim of this research is to clone mic3 (gene encoding for MIC-3) of T. gondii from local isolate using recombinant DNA technology by cloning mic3 in an expression vector. Deoxyribonucleic acid (DNA) from T. gondii tachyzoites was amplified by PuRe Taq RTG-PCR Beads using mic3 specific primers. Amplified DNA was double digested using EcoRV and HindIII restriction endonucleases and then purified using EZ-10 spin coloumn purification kit. The mic3 DNA was ligated into pET-32a(+) expression vector and transformated into Escherichia coli BL21. The results showed that recombinant mic3 gene 4.2 kDa has been successfully performed by cloning gene encoding for MIC-3 protein of T. gondii local isolate into pET-32a(+) and transformed to E. coli BL21. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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