Loss of GRB2 associated binding protein 1 in arteriosclerosis obliterans promotes host autophagy
| Autor: | Meng Ye, Xiang-Jiang Guo, Ke-Jia Kan, Qi-Hong Ni, Jia-Quan Chen, Han Wang, Xin Qian, Guan-Hua Xue, Hao-Yu Deng, Lan Zhang, Peng Lyu |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Chinese Medical Journal, Vol 134, Iss 1, Pp 73-80 (2021) |
| Druh dokumentu: | article |
| ISSN: | 0366-6999 2542-5641 00000000 |
| DOI: | 10.1097/CM9.0000000000001255 |
| Popis: | Abstract. Background:. Arteriosclerosis obliterans (ASO) is a major cause of adult limb loss worldwide. Autophagy of vascular endothelial cell (VEC) contributes to the ASO progression. However, the molecular mechanism that controls VEC autophagy remains unclear. In this study, we aimed to explore the role of the GRB2 associated binding protein 1 (GAB1) in regulating VEC autophagy. Methods:. In vivo and in vitro studies were applied to determine the loss of adapt protein GAB1 in association with ASO progression. Histological GAB1 expression was measured in sclerotic vascular intima and normal vascular intima. Gain- and loss-of-function of GAB1 were applied in VEC to determine the effect and potential downstream signaling of GAB1. Results:. The autophagy repressor p62 was significantly downregulated in ASO intima as compared to that in healthy donor (0.80 vs. 0.20, t = 6.43, P |
| Databáze: | Directory of Open Access Journals |
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