| Autor: |
J. M. Robledo Verduzco, José Herrera Camacho, M. Cajero Juaréz, M. C. Navarro Maldonado, A. GarcÃa Valladares |
| Jazyk: |
English<br />Spanish; Castilian |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
Tropical and Subtropical Agroecosystems, Vol 10, Iss 1, Pp 95-99 (2008) |
| Druh dokumentu: |
article |
| ISSN: |
1870-0462 |
| Popis: |
The aim of this work was to evaluate the effect of HECM-9 and TCM-199 as maturation media on maturation (MR), in vitro fertilization (IVF) and embryo development (ED) rate of oocytes from hair sheep collected from slaughter house ovaries. Cumulus-oocyte-complexes (COC) were obtained by manual aspiration, from 2-6mm diameter follicles. Groups of 10-20 COC, quality 1 and 2 were placed into 450μL of HECM-9 or TCM-199 and incubated 24h at 38.5 ºC and 5% CO2. For IVF, COC were transferred to 450μL of SOFm+Oaa, 0.5 x 106 motile spermatozoa were added and then incubated at 38.5 ºC and 5% CO2. Alleged zygotes were transferred to 450μL of SOFm+Oaa+glucose. Embryo development and morphology were evaluated at 2, 4, 5 and 6 days of culture, not developed zygotes were removed on day 2 and the final rate of embryo production was determined on day 8 of culture. Oocyte MR showed no significant differences (P>0.05) between treatments (73.3 vs. 71.4 % HECM-9 and TCM-199, respectively). Fertilization rate was different (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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