Detection of Epstein Barr virus in formalin-fixed paraffin tissues by fluorescent direct in situ PCR
| Autor: | N Marziliano, E Arbustini, M Rossi de Gasperis, S Crovella |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | European Journal of Histochemistry, Vol 49, Iss 3 (2009) |
| Druh dokumentu: | article |
| ISSN: | 1121-760X 2038-8306 |
| DOI: | 10.4081/958 |
| Popis: | Specific viral laboratory diagnosis of primary Epstein-Barr Virus (EBV) infection is usually based on antibody-detection assays. However, molecular detection is also considered the reference standard assay for diagnosis of central nervous system infections and of most cases of nasopharyngeal carcinoma (NPC). One-step or nested polymerase chain reaction (PCR) has rapidly replaced immunological assays based on virus-specific Ig antibodies for the laboratory diagnosis of Herpesvirus infections, even if serological methods are considered an additional tool for defining clinical diagnosis. In this article, we will present a rapid, sensitive and robust molecular tool for the viral detection of EBV (EBNA-1) within tissue specimens by making use of in situ PCR (IS-PCR). |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|