Autor: |
Xiaohui Wu, Mengyuan Zhang, Zheng Zheng, Ziqi Sun, Feiyan Qi, Hua Liu, Juan Wang, Mengmeng Wang, Ruifang Zhao, Yue Wu, Xiao Wang, Hongfei Liu, Wenzhao Dong, Xinyou Zhang |
Jazyk: |
angličtina |
Rok vydání: |
2024 |
Předmět: |
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Zdroj: |
Journal of Integrative Agriculture, Vol 23, Iss 5, Pp 1494-1506 (2024) |
Druh dokumentu: |
article |
ISSN: |
2095-3119 |
DOI: |
10.1016/j.jia.2023.10.036 |
Popis: |
Peanut (Arachis hypogaea L.) is a globally important oil crop. Web blotch is one of the most important foliar diseases affecting peanut, which results in serious yield losses worldwide. Breeding web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web blotch. In the current study, a bulked segregant analysis with next-generation sequencing was used to analyze an F2:3 segregating population and identify candidate loci related to web blotch resistance. Based on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR (KASP) markers, we identified a novel web blotch resistance-related locus spanning approximately 169 kb on chromosome 16. This region included four annotated genes, of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two parents. Two markers (Chr.16.12872635 and Chr.16.12966357) linked to this gene were shown to be co-segregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines (RIL), which could be used in marker-assisted breeding of resistant peanut varieties. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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