Fine-mapping of a candidate gene for web blotch resistance in Arachis hypogaea L.

Autor: Xiaohui Wu, Mengyuan Zhang, Zheng Zheng, Ziqi Sun, Feiyan Qi, Hua Liu, Juan Wang, Mengmeng Wang, Ruifang Zhao, Yue Wu, Xiao Wang, Hongfei Liu, Wenzhao Dong, Xinyou Zhang
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Journal of Integrative Agriculture, Vol 23, Iss 5, Pp 1494-1506 (2024)
Druh dokumentu: article
ISSN: 2095-3119
DOI: 10.1016/j.jia.2023.10.036
Popis: Peanut (Arachis hypogaea L.) is a globally important oil crop. Web blotch is one of the most important foliar diseases affecting peanut, which results in serious yield losses worldwide. Breeding web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web blotch. In the current study, a bulked segregant analysis with next-generation sequencing was used to analyze an F2:3 segregating population and identify candidate loci related to web blotch resistance. Based on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR (KASP) markers, we identified a novel web blotch resistance-related locus spanning approximately 169 kb on chromosome 16. This region included four annotated genes, of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two parents. Two markers (Chr.16.12872635 and Chr.16.12966357) linked to this gene were shown to be co-segregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines (RIL), which could be used in marker-assisted breeding of resistant peanut varieties.
Databáze: Directory of Open Access Journals