A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia

Autor:	Stephanie Wagner Gallo, Patricia Locosque Ramos, Carlos Alexandre Sanchez Ferreira, Silvia Dias de Oliveira
Jazyk:	angličtina
Rok vydání:	2013
Předmět:	Stenotrophomonas maltophilia identification PCR Microbiology QR1-502 Infectious and parasitic diseases RC109-216
Zdroj:	Memorias do Instituto Oswaldo Cruz, Vol 108, Iss 3, Pp 390-391 (2013)
Druh dokumentu:	article
ISSN:	1678-8060 0074-0276
DOI:	10.1590/S0074-02762013000300020
Popis:	Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/d455b0668e454883a1ef9bc0c2684899 Zobrazit plný text záznamu View record in DOAJ