Cryopreservation affects platelet macromolecular composition over time after thawing and differently impacts on cancer cells behavior in vitro

Autor: Gaia Gavioli, Agnese Razzoli, Diana E. Bedolla, Erminia Di Bartolomeo, Eleonora Quartieri, Barbara Iotti, Pamela Berni, Giovanni Birarda, Lisa Vaccari, Davide Schiroli, Chiara Marraccini, Roberto Baricchi, Lucia Merolle
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Platelets, Vol 34, Iss 1 (2023)
Druh dokumentu: article
ISSN: 09537104
1369-1635
0953-7104
DOI: 10.1080/09537104.2023.2281943
Popis: AbstractCryopreservation affects platelets’ function, questioning their use for cancer patients. We aimed to investigate the biochemical events that occur over time after thawing to optimize transfusion timing and evaluate the effect of platelet supernatants on tumor cell behavior in vitro. We compared fresh (Fresh-PLT) with Cryopreserved platelets (Cryo-PLT) at 1 h, 3 h and 6 h after thawing. MCF-7 and HL-60 cells were cultured with Fresh- or 1 h Cryo-PLT supernatants to investigate cell proliferation, migration, and PLT-cell adhesion. We noticed a significant impairment of hemostatic activity accompanied by a post-thaw decrease of CD42b+ , which identifies the CD62P−-population. FTIR spectroscopy revealed a decrease in the total protein content together with changes in their conformational structure, which identified two sub-groups: 1) Fresh and 1 h Cryo-PLT; 2) 3 h and 6 h cryo-PLT. Extracellular vesicle shedding and phosphatidylserine externalization (PS) increased after thawing. Cryo-PLT supernatants inhibited cell proliferation, impaired MCF-7 cell migration, and reduced ability to adhere to tumor cells. Within the first 3 hours after thawing, irreversible alterations of biomolecular structure occur in Cryo-PLT. Nevertheless, Cryo-PLT should be considered safe for the transfusion of cancer patients because of their insufficient capability to promote cancer cell proliferation, adhesion, or migration.
Databáze: Directory of Open Access Journals
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