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Objective: This study was conducted to evaluate the effect of brewer's spent yeast (BSY) inclusion level and ensiling duration (ED) on fermentative, fungal load dynamics, and nutritional characteristics of brewer's spent-yeast based silage. Materials and methods: To prepare the silages materials, 4 BSY inclusion levels (0, 10, 20, and 30%) to replace BSG and 3 ED (2,4 and 6 weeks) were arranged in 4 × 3 factorial combination using a completely randomized design (CRD) in 5 replications. The ratio of brewery spent grain (BSG) to wheat bran (WB) used majorly as protein and energy sources, respectively was 30:69 with a 1% salt addition. Parameters measured include observation for surface spoilage, yeast and mold colony count, silage temperature, pH, total dry matter loss (TDML), major proximate, detergent fractions and permanganate lignin, in-vitro organic matter digestibility (IVOMD) and estimated metabolizable energy (EME) values. Results: The study revealed that at any BSY inclusion level and ED, extensive mold growths and discolorations were not observed. However, slightly higher values of 6.5, 5.7, and 12.2 colonies forming unit (CFU)/g DM yeast, mold, and total fungal counts (TFC), respectively were recorded only at the 6 weeks of the fermentation period with 30% BSY inclusion level. Brewer's spent yeast inclusion level and ED had a significant (P |
