Development of a SYBR Green-Based RT-qPCR Assay for the Detection and Quantification of Lone Star Virus
| Autor: | Megan Burch, Jeremy Bechelli |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Zoonoses, Vol 4, Iss 1, p 984 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2737-7474 2737-7466 |
| DOI: | 10.15212/ZOONOSES-2023-0037 |
| Popis: | Lone Star virus (LSV) is a newly characterized arbovirus with pathogenic potential. However, no detection methods are available to specifically identify and monitor LSV. Two SYBR green-based RT-qPCRs were developed and validated for the detection and quantification of LSV. Two primer sets were evaluated for reproducibility, specificity, and sensitivity. The primer sets were applied to monitor viral titers in vitro and via surveillance of LSV in collected ticks. One primer set (LSV-S) was the most specific for LSV when tested against 11 other vector-borne viruses. While less specific, the LSV-M primer set detected |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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