Otimização e validação de método multirresíduo para determinação de sulfonamidas em camarão cultivado por cromatografia líquida de alta eficiência com detecção por UV
| Autor: | Paloma Nascimento dos Santos, Maria Inês Sucupira Maciel, Beatriz Regina Brito de Oliveira Lavorante, Marcelo de Mélo Medeiros, Eden Cavalcanti de Albuquerque Júnior |
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| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Química Nova, Vol 34, Iss 7, Pp 1265-1270 (2011) |
| Druh dokumentu: | article |
| ISSN: | 1678-7064 0100-4042 |
| DOI: | 10.1590/S0100-40422011000700027 |
| Popis: | This work reports the optimization and method validation for sulfonamides (sulfamethazine, sulfaquinoxaline, sulfadimethoxine and sulfathiazole) in shrimp muscle using HPLC-UV. The sulfonamides were extracted with acetonitrile and acetic acid, and the extract cleaned up with a Strata SCX SPE cartridge prior to analysis. The method presented linearity in the range of 20-120 µg kg-1, good linear correlation (r > 0.99), and limits of quantification in the range of 4.7-20.2 µg kg-1. The recovery for shrimp muscles spiked with 50-150 µg kg-1 ranged from 63.2-108.0%. Precision and accuracy analysis showed acceptable relative standard deviation. Commercial shrimps were analyzed and sulfonamides don't were found above of the method limit of quantification. |
| Databáze: | Directory of Open Access Journals |
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