| Autor: |
Cotrim Paola, Andrade Cleverton Roberto de, Line Sergio, Almeida Oslei Paes de, Coletta Ricardo D. |
| Jazyk: |
angličtina |
| Rok vydání: |
2002 |
| Předmět: |
|
| Zdroj: |
Brazilian Dental Journal, Vol 13, Iss 2, Pp 97-102 (2002) |
| Druh dokumentu: |
article |
| ISSN: |
0103-6440 |
| Popis: |
Tooth germ development is associated with morphological and biochemical changes of the dental papilla and enamel organ. Enzymes with gelatinolytic activities were studied by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzymography in tooth germ of newborn to 15-day-old rats. Three major bands with gelatinolytic activity were detected at all periods and characterized as the latent and active forms of MMP-2 using their molecular weight and activity dependent on Zn++ and Ca++ ions as criteria. Expression and activity of MMP-2 increased progressively from 0 to 15 days after birth. Mechanical separation of the tooth germ from 10-day-old rats showed that the gelatinolytic activity was localized mainly in the dental papilla and not the dental organ. These data indicate that the expression and activity of MMP-2 varies during the development and maturation of rat first molar tooth germ. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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