| Autor: |
John Kaldor, Steven G Badman, Josephine Gabuzzi, Suzanne Garland, Julia Brotherton, Monica Molano, Samuel Phillips, David Hawkes, Dorothy A Machalek, Grace Tan, Zure Kombati, Gloria Munnull, Marion Saville, Gerald L Murray, John Bolnga, Andrew John Vallely, Prisha Balgovind, Gholamreza Haqshenas, Alyssa Marie Cornall, Pamela Josephine Toliman |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
BMJ Open, Vol 14, Iss 6 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2044-6055 |
| DOI: |
10.1136/bmjopen-2023-081282 |
| Popis: |
Objective WHO recommends human papillomavirus (HPV) testing for cervical screening, with triage of high-risk HPV (hrHPV) positive women. However, there are limitations to effective triage for low-resource, high-burden settings, such as Papua New Guinea. In this exploratory study, we assessed the performance of host methylation as triage tools for predicting high-grade squamous intraepithelial lesions (HSIL) in self-collected and clinician-collected samples.Design Exploratory observational study.Setting Provincial hospital, same-day cervical screen-and-treat trial, Papua New Guinea.Participants 44 hrHPV+women, with paired self/clinician-collected samples (4 squamous cell carcinomas (SCC), 19 HSIL, 4 low-grade squamous intraepithelial lesions, 17 normal).Primary and secondary outcome measures Methylation levels of CADM1, MAL and miR124-2 analysed by methylation-specific PCRs against the clinical endpoint of HSIL or SCC (HSIL+) measured using liquid-based-cytology/p16-Ki67 stain.Results In clinician-collected samples, MAL and miR124-2 methylation levels were significantly higher with increasing grade of disease (p=0.0046 and p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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