Influence of Organic Matter from Native Fish on the Antimicrobial Efficacy of Sodium Hypochlorite (NaClO) in Reducing Salmonella spp. Population

Autor: Jaqueline Oliveira Reis, Carine Baggio Cavalcante, Nathaly Barros Nunes, Adelino Cunha Neto, Maxsueli Aparecida Moura Machado, Yuri Duarte Porto, Vinicius Silva Castro, Eduardo Eustáquio de Souza Figueiredo
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Microbiology Research, Vol 15, Iss 1, Pp 342-353 (2024)
Druh dokumentu: article
ISSN: 15010023
2036-7481
DOI: 10.3390/microbiolres15010023
Popis: Salmonella spp. is not part of the fish microbiota, being introduced through contaminated water or improper handling. In slaughterhouses, five parts per million (ppm) of free chlorine is recommended in the washing step to eliminate this pathogen. However, Salmonella spp. is still detected in fish processing plants due to persistent contamination as a result of chlorine–organic matter interactions that reduce chlorine effectiveness. Therefore, this study aimed to test whether organic matter contained in culture media and fish washes interferes with the efficacy of chlorine at 2 and 5 ppm, and what is the time of action required to inactivate ATCC and wild strains of S. typhimurium. For this, the elimination or survival of these strains was investigated when exposed to chlorinated solution for different durations (0, 5, 30, 60, 90 and 120 min) in culture medium, 0.85% saline solution and fish wash (in natura and sterilized). The results showed that the use of 5 ppm of free chlorine is efficient, even when the chlorine remains in contact with the organic matter (washed from the fish), as it reduced the bacterial population by ~5 log log10 CFU/mL of Salmonella spp., showing that this reduction was due to interactions, as long as the chlorine action time was 30 min.
Databáze: Directory of Open Access Journals
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