In vitro antimicrobial activity of methanolic extracts from cutaneous secretions of Amazonian amphibians against phytopathogens of agricultural interest

Autor: Livia Deice RAASCH-FERNANDES, Solange Maria BONALDO, Domingos de Jesus RODRIGUES, Stela Regina FERRARINI, Ana Gabriela Araújo VERÇOSA, Daiane Lopes de OLIVEIRA
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2021
Předmět:
Zdroj: Acta Amazonica, Vol 51, Iss 2, Pp 145-155 (2021)
Druh dokumentu: article
ISSN: 0044-5967
1809-4392
DOI: 10.1590/1809-4392201904462
Popis: ABSTRACT The biochemical defense mechanisms of amphibians involve cutaneous secretions of bioactive molecules with antimicrobial activity. This study evaluated the in vitro activity of methanolic extracts from cutaneous secretions of two amphibian species of the Bufonidae family, Rhaebo guttatus and Rhinella marina, in the control of the phytopathogens Fusarium udum, Fusarium solani, Colletotrichum truncatum, Aspergillus flavus, Rhizoctonia solani, Macrophomina phaseolina, and Calonectria pseudometrosideri. The R. guttatus extract decreased the mycelial growth of F. udum, F. solani, A. flavus, and M. phaseolina at some tested concentrations. The R. marina extract decreased the mycelial growth of C. truncatum at the concentration of 0.5 mg mL-¹, and inhibited the mycelial growth of A. flavus at concentrations of 0.1 and 0.5 mg mL-¹, which was similar to the inhibition by the positive control. The R. marina extract also decreased the microsclerotia production by R. solani at concentrations of 0.2 and 0.3 mg mL-¹. In addition, the extracts inhibited conidial sporulation and germination at varying degrees. The inhibition of appressoria formation in C. truncatum by the R. guttatus and R. marina extracts was 85-99% and 63-100%, respectively. Our results demonstrated that treatment with extracts from R. guttatus and R. marina cutaneous secretions showed antifungal activity against the studied phytopathogens.
Databáze: Directory of Open Access Journals
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